Gonyaulax grindleyi P. Reinecke (redirected from: Protoceratium reticulatum)
Family: Gonyaulacaceae
Synonyms: Protoceratium reticulatum (Clap. and J. Lachm.) Buetschli
Gonyaulax grindleyi image
Protoceratium reticulatum  

Species Description: This dinoflagellate exists as unicells with an oval shape (Figures 1, 2 & 5), occasionally becoming somewhat polygonal. The cingulum is displaced about one cingular width circumferentially (Figures 2 & 5), and is located anterior to the cell midpoint, making the hypotheca larger than the epitheca (Figures 1 & 2). The sulcus does not reach the antapex (Figures 2 & 5). The cell surface is highly reticulate, which makes the plate pattern difficult to evaluate (Figures 1, 4 & 5). The first apical plate (1’) has a ventral pore on its left side (Figures 2, 4 & 6). Both Steidinger & Tangen (1997) and Throndsen et al. (2007) give a plate formula for the genus as Po,3’, 0a, 6’’, 6c, 6s, 6’’’, 0p,2’’’’. The hypnocyst (Figure 3) is spherical or subspherical and hyaline, with a smooth cyst wall and ornamented with 20-40 slender spines. Spines have capitate ends (Figure 3) and are about ¼ the length of the cyst diameter, though with some variation in length depending on salinity and temperature (Mertens et al. 2010).

There are numerous brown chloroplasts, which often obscure the plates. The longitudinal (trailing) flagellum does not extend very far beyond the antapex. Additional characterizations of morphological variability are found in Hansen et al. (1997) and Hernandez-Becerril et al. (2010). The relationship of P. reticulatum to other toxin producing species, based on molecular genetics, is found in Howard et al. (2009) and Riccardi et al. (2006).

Habitat & Regional Occurrence: Nearly all records of occurrence are coastal or estuarine. Because of its shape and cryptic plates (due to the reticulation), P. reticulatum is fairly easy to recognize, particularly if cysts are also seen. It is very widely distributed in temperate and tropical areas, and has been recorded from sub-boreal locations in summer (Throndsen et al. 2007). It has also been recorded in the North and South Atlantic, as well as North and South Pacific, and seas associated with the Indian Ocean (e.g. Red Sea). Some older records of its occurrence use Reinecke’s name, Gonyaulax grindleyi.

Indian River Lagoon Distribution: In the IRL, this species occurs most often in summer and fall, though apparently not in significant numbers. It is more common in the central IRL than in the northern parts.

Size: Vegetative cells are 28-53 µm in apical length and 25-45 µm in width (literature records). The cysts are about 30-40 µm in diameter.

Reproduction & Abundance: Details of the life history have not been adequately investigated. This species is widespread and fairly common throughout the IRL, but rarely in bloom proportions (e.g. more than 106 cells per liter). The hypnocysts have not been verified from the IRL, though they are present on Florida’s west coast. Blooms have been reported from South Africa (as G. grindleyi, Reinicke 1967) and New Zealand.

Toxicity: P. reticulatum is a cause of extensive fish kills due to a group of lipophilic sulphated polyether toxins called yessotoxins (YTX), named after Patinopecten yessoensis, the mollusk in which they were first identified. Yessotoxins were first assumed to be a cause of diarrhetic shellfish poisoning (Paz et al. 2008), but their chemical structure and biological mode of action is different. Although human toxicity of YTZ and its 40+ analogs are unknown, the occurrence of P. reticulatum has been of concern in shellfish aquaculture (Paz et al. 2008). Some shellfish aquaculture facilities have reportedly closed for long periods due to yessotoxins in the shellfish (Guerini et al. 2007). Experiments with zooplankton and field observations have demonstrated the inhibitory properties of yessotoxins on growth, reproduction, and mortality of marine animals (Horstman 1981; Huntley et al. 1986; Makino et al. 2008).

Toxin production appears to be dependent on geographic location and growth conditions (Gallardo Rodríguez et al. 2010). Guerrini et al. (2007) demonstrated toxin increases under P-limitation, but N-limitation produced toxin levels similar to controls. Moreover, their strains of P. reticulatum grew at salinities of 22-42 PSU and temperatures of 16-26°C. At present no toxin production has been confirmed from IRL strains. Bioluminescence is apparently strain specific and uncommon, but has been reported in some strains (Poupin et al. 1999).

Gallardo Rodríguez, JJ, Sanchez Mirón, A, García Camacho, F, Cerón Garcia, MC, Belarbi, EH & E Molina Grima. 2010. Culture of dinoflagellates in a fed-batch and continuous stirred-tank photobioreactors: Growth, oxidative stress and toxin production. Process Biochem. 45: 660-666.

Guerrini, F, Ciminello, P, Dell’Aversano, C, Tartagione, L, Fattorusso, E, Boni, L & R Pistocchi. 2007. Influence of temperature, salinity and nutrient limitation on yessotoxin production and release by the dinoflagellate Protoceratium reticulatum in batch cultures. Harmful Algae 6: 707-717.

Hansen, G, Moestrup, O & KR Roberts. 1997. Light and electron microscopical observations on Protoceratium reticulatum (Dinophyceae). Archiv für Protistenkunde 147: 381-391.

Hernandez-Becerril, DU, Rodriguez-Palacio, MC & C Lozano-Ramirez. 2010. Morphology of two bloom-forming or potentially toxic marine dinoflagellates from the Mexican Pacific, Heterocapsa pymaea and Protoceratium reticulatum (Dinophyceae). Cryptogamie: Algologie 31: 245-254.

Horstman, DA. 1981. Reported red water outbreaks and their effects on fauna of the west and south coasts of South Africa. Fish. Bull. S. Africa 15: 71-88.

Howard, M, Smith, GJ & RM Kudela. 2009. Phylogenetic relationships of yessotoxin-producing dinoflagellates, based on the large subunit and internal transcribed spacer ribosomal DNA domains. Appl. & Env. Microbiol. 75: 54-63.

Huntley, M, Sykes, P, Rohan, S & V Martin. 1986. Chemically-mediated rejection of dinoflagellate prey by the copepods Calanus pacificus and Paracalanus parvus: mechanism, occurrence and significance. Mar. Ecol. Prog. Ser. 28: 105-120.

Makino, W, Ito, K, Oshima, Y & J Urabe. 2008. Effects of Protoceratium reticulatum yessotoxin on feeding rates of Acartia hudsonica: A bioassay using artificial particles coated with purified toxin. Harmful Algae 7: 639-645.

Matsuoka, K, McMinn, A & JH Wrenn. 1997. Restudy of the holotype of Operculodinium centrocarpum (Deflandre & Cookson) Wall (Dinophyceae) from the Miocene of Australia, and the taxonomy of related species. Palynology 21: 19-33.

Mertens, KN, Dale, B, Ellegaard, M, Jansson, I-M, Godhe, A, Kremp, A & S Louwye. 2010: Process length variation in cysts of the dinoflagellate Protoceratium reticulatum, from surface sediments of the Baltic–Kattegat–Skagerrak estuarine system: a regional salinity proxy. Boreas, 10.1111/j.1502-3885.2010.00193.x. ISSN 0300-9483.

Paz, B, Daranas, AH, Norte, M, Riobó, P, Franco, J & JJ Fernández. 2008. Yessotoxins, a group of marine polyether toxins: An overview. Mar. Drugs 6: 73-102.

Poupin, J, Cussatlegras, A-S & P Geistdoerfer. 1999. Plancton marin bioluminescent inventaire documenté des espècies et bilan des formes les plus communes de la mer d’Iroise. Laboratoire d’Oceanographie de l’Ecole Navale, LOEN Lanvéc-Poulmic, France. 1-83.

Reinecke, P. 1967. Gonyaulax grindleyi sp. nov.: A dinoflagellate causing a red tide at Elands Bay, Cape Province, in December 1966. J. S. African Bot. 33: 157-160.

Riccardi, M, Guerrini, F, Pistocchi, R, Tinti, F & L Boni. 2006. Molecular characterization of different strains of Protoceratium reticulatum (Claparede & Lachmann) Buetschli. Biologia marina mediterranea 13: 1042-1044.

Steidinger, KA & K Tangen. 1997. Dinoflagellates.387-584 In: Tomas, C. (Ed.) Identifying Marine Phytoplankton. Academic Press Inc., San Diego,

CA. Stosch, HA von. 1969. Dinoflagellaten aus der Nordsee I. Über Cachonina niei Loeblich (1968), Gonyaulax grindleyi Reinecke (1967) und eine Methode zur Darstellung von Peridineenpanzern. Helgoländer wissenschaftliche Meeresuntersuchungen 19: 558-568.

Throndsen, J, Hasle, GR & K Tangen. 2007. Phytoplankton of Norwegian Coastal Waters. Almater Forlag AS, Oslo, Norway. 343 pp.

Antapex: Posterior-most part of the cell body, excluding spines, lists and similar structures.

Anterior Intercalary Plates: Plates located between the apical and precingular plates.

Apex: Anterior-most part of the cell body.

Apical Groove: Groove located at the anterior part of many dinoflagellate species, extending posteriorly on both the ventral and dorsal surfaces of the cell; also known as the acrobase.

Apical Plates: Plates that surround and touch the cell apex.

Apical Pore Complex: A pore or hole at the cell apex that may have one or more tiny accessory plates; sometimes abbreviated as ‘APC’.

Areolate: Ornamentation of the thecal plates that consists of depressions of variable depth and form.

Cingular Plates: Plates that are located in the cingular, or girdle, groove around the cell.

Cingulum: A furrow encircling the cell that contains the rotatary flagellum.

Cyst: The diploid zygotic dormant stage in the sexual life cycle; usually morphologically dissimilar from the haploid motile stage; also called the ‘dinocyst’ or ‘hypnozygote’.

Dorsal Side: Back side of the cell, opposite of the front ventral side where the sulcus is located.

Epicone: The part of a dinoflagellate cell above the cingulum; usually refers to an ‘unarmored’ (lacking cellulose plates) cell; may also be known as the epitheca or episome.

Epitheca: The part of a dinoflagellate cell above the cingulum; usually refers to a thecate (with cellulose plates) cell; may also be known as the epicone or episome.

Flagellar Area: The vicinity of the origin of the two flagella.

Hypocone: The part of a dinoflagellate cell below the cingulum; usually refers to an ‘unarmored’ (lacking cellulose plates) cell; may also be referred to as the hypotheca or hyposome.

Hypotheca: The part of a dinoflagellate cell below the cingulum; usually refers to a thecate (with cellulose plates) cell; may also be referred to as the hypocone or hyposome.

Lists: Membranous extensions of the cingulum and/or sulcus that extend beyond the cell wall boundary; found in thecate dinoflagellates, especially those from the order Dinophysiales.

Median Cingulum: A cingulum that encircles approximately the midpoint of the cell.

Nematocyst: Ejectile organelle of some dinoflagellate genera; involved in prey capture.

Peduncle: Cytoplasmic appendage near the flagellar pores; organelle associated with phagotrophy.

Pores: Openings in the theca that can be involved in the extrusion of certain structures from the cell; genetically variable and used for identification of species; also known as trichocyst pores.

Postcingular Plates: Plates located immediately below the cingulum.

Posterior Intercalary Plates: Plates located between the postcingular and antapical plates.

Postmedian Cingulum: A cingulum that encircles the cell below the midpoint.

Precingular Plates: Plates located immediately above the cingulum.

Premedian Cingulum: A cingulum that encircles the cell above the midpoint.

Ribs: Supports for sulcal lists.

Sulcal Plates: Plates located in the sulcus.

Sulcus: A longitudinal furrow, often partially enclosing the propulsive flagellum.

Thecate: One of many dinoflagellates having a cell wall of cellulose plates, which have special designations and symbols according to their location on the cell. Number and location of thecal plates is often genus-specific.

Gonyaulax grindleyi image
Protoceratium reticulatum  
Gonyaulax grindleyi image
Protoceratium reticulatum  
Gonyaulax grindleyi image
Protoceratium reticulatum  
Gonyaulax grindleyi image
Protoceratium reticulatum  
Gonyaulax grindleyi image
Protoceratium reticulatum  
Gonyaulax grindleyi image
Protoceratium reticulatum